Biochem/physiol Actions

Cell permeable: no

Reversible: no

Primary TargetFluorogenic caspase-1 substrate

Product does not compete with ATP.

General description

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jones, R.A., et al. 1998. Hepatology 27, 1632.Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Walker, N.P., et al. 1994. Cell 78, 343.Thornberry, N.A., et al. 1992. Nature 356, 768.

Packaging

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Ac-Tyr-Val-Ala-Asp-AFC

Warning

Toxicity: Standard Handling (A)